| 產(chǎn)品編號 | BIO0993SM |
| 英文名稱 | Anti-CD20 & CD3 Reference Antibody (Odronextamab Biosimilar) |
| 別 名 | CD20 & CD3; Odronextamab |
| 抗體來源 | |
| 克隆類型 | Monoclonal |
| 交叉反應 | Human |
| 產(chǎn)品應用 |
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 145.57kDa |
| 性 狀 | Lyophilized |
| 亞 型 | IgG-like |
| 純化方法 | Protein A |
| 緩 沖 液 | 100 mM Pro-Ac 20mM Arg pH 5.0 |
| 保存條件 | -80°C for 2 years under sterile conditions； -20°C for 1 year under sterile conditions；Avoid repeated freeze-thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | |
| 產(chǎn)品圖片 |
Co-incubation of Odronextab with Jurkat cells, then with the addition of Raji cells for 6 hours. Bright-Lite was used to detect the fluorescent signal.
As shown in fig, Odronextamab was able to activate the NF-AT signaling pathway.
Odronextamab bound to huCD3e-jurkat cells, and then rebounded to fluorescent secondary antibodies(Anti-Human IgG, Fcγ PE) , and test by flow cytometry. As shown in fig, Odronextamab bound to huCD3e-jurkat cells, and the EC50 was 26.520 nM.
Odronextamab bound to Raji cells, and then rebounded to fluorescent secondary antibodies(Anti-Human IgG, Fcγ PE), and test by flow cytometry. As shown in fig, Odronextamab bound to Raji cells, and the EC50 was 17.3 nM.
Odronextamab bound to CD20 protein, and then rebounded to secondary antibodies(Anti-Human-IgG-Fc-HRP) , and read OD450. As shown in fig, Odronextamab bound human CD20-VLP, and the EC50 was 0.1964 nM.
The purity of Anti-CD20 & CD3 Reference Antibody (Odronextamab) is 97.48%, determined by SEC-HPLC.
Anti-CD20 & CD3 Reference Antibody (Odronextamab) on SDS-PAGE under reducing (R) condition. The purity of the protein is greater than 95%.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
