| 產(chǎn)品編號 | BIO1006SM |
| 英文名稱 | Anti-B7-H3 & EGFR Reference Antibody (Ibi-334 Biosimilar) |
| 別 名 | B7-H3 / CD276 & ERBB1 / EGFR / HER1; Ibi-334 |
| 抗體來源 | |
| 克隆類型 | Monoclonal |
| 交叉反應(yīng) | Human |
| 產(chǎn)品應(yīng)用 |
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 145.58kDa |
| 性 狀 | Lyophilized |
| 亞 型 | IgG-like |
| 純化方法 | Protein A |
| 緩 沖 液 | 100 mM Pro-Ac 20mM Arg pH 5.0 |
| 保存條件 | -80°C for 2 years under sterile conditions； -20°C for 1 year under sterile conditions；Avoid repeated freeze-thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | |
| 產(chǎn)品圖片 |
Ibi-334 bound to in huEGFR CHO-K cells, and then rebounded to fluorescent secondary antibodies(Anti-Human IgG, Fcγ PE) , and test by flow cytometry. As shown in fig, Ibi-334 bound to in huEGFR CHO-K cells, and the EC50 was 5.398 nM.
Ibi-334 bound to huB7-H3 CHO-K cells, and then rebounded to fluorescent secondary antibodies(Anti-Human IgG, Fcγ PE) , and test by flow cytometry. As shown in fig, Ibi-334 bound to huB7-H3 CHO-K cells, and the EC50 was 4.066 nM.
Ibi-334 bound to ERBB1/EGFR/HER1 protein, and then rebounded to secondary antibodies(Anti-Human-IgG-Fc-HRP) , and read OD450. As shown in fig, Ibi-334 bound human ERBB1/EGFR/HER1 Protein-His, and the EC50 was 0.029 nM.
Ibi-334 bound to B7-H3 protein, and then rebounded to secondary antibodies (Anti-Human-IgG-Fc-HRP) , and read OD450. As shown in fig, Ibi-334 bound human B7-H3 Protein-His, and the EC50 was 0.033 nM.
The purity of Anti-B7-H3 & EGFR Reference Antibody (Ibi-334) is 99.17%, determined by SEC-HPLC.
Anti-B7-H3 & EGFR Reference Antibody (Ibi-334) on SDS-PAGE under reducing (R) condition. The purity of the protein is greater than 95%.
The detected molecular weight of Anti-EGFR & B7-H3 Reference Antibody (Ibi-334) is 145.31 kDa.
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| 1、抗體溶解方法 | |
| 2、抗體修復(fù)方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關(guān)于肽鏈的設(shè)計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
