| 產(chǎn)品編號 | BIO0971SM |
| 英文名稱 | Anti-CD47 & PD-1 Reference Antibody (Hx009 Biosimilar) |
| 別 名 | CD47 & PDCD1 / PD-1 / CD279; Hx009 |
| 抗體來源 | |
| 克隆類型 | Monoclonal |
| 交叉反應 | Human |
| 產(chǎn)品應用 |
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 173.16kDa |
| 性 狀 | Lyophilized |
| 亞 型 | IgG-VHH |
| 純化方法 | Protein A |
| 緩 沖 液 | 100 mM Pro-Ac 20mM Arg pH 5.0 |
| 保存條件 | -80°C for 2 years under sterile conditions； -20°C for 1 year under sterile conditions；Avoid repeated freeze-thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 | |
| 產(chǎn)品圖片 |
Hx009 bound to huPD-1-Jurkat cells, and then rebounded to fluorescent secondary antibodies(Anti-Human IgG, Fcγ PE) , and test by flow cytometry. As shown in fig, Hx009 bound to huPD-1-Jurkat cells, and the EC50 was 0.747 nM.
Hx009 bound to PD-1 protein, and then rebounded to secondary antibodies(Anti-Human-κ+λ-HRP) , and read OD450. As shown in fig, Hx009 bound to PD-1-FC, and the EC50 was 0.030 nM.
Hx009 bound to CD47 protein, and then rebounded to secondary antibodies(Anti-Human-IgG-Fc-HRP) , and read OD450. As shown in fig, Hx009 bound to CD47-His, and the EC50 was 0.364 nM.
The purity of Anti-CD47 & PD-1 Reference Antibody (Hx009）is 100%, determined by SEC-HPLC.
Anti-CD47 & PD-1 Reference Antibody (Hx009）on SDS-PAGE under reducing (R) condition. The purity of the protein is greater than 95%.
The detected molecular weight of Anti-CD47 & PD-1 Reference Antibody (Hx009) is 173.16 kDa.
|
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
