| 產(chǎn)品編號 | bsm-33037R-HRP |
| 英文名稱 | COX4I1 Recombinant Rabbit mAb, HRP c |
| 中文名稱 | HRP標記的細胞色素c氧化酶IV亞型1（內參）重組兔單抗 |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 交叉反應 | Human, Mouse, Rat |
| 產(chǎn)品應用 | IHC-P=1:100-500, IHC-F=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human COX4I1 Recombinant Rabbit mAb, HRP c |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it. [provided by RefSeq, Jul 2008] |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The?section?was?incubated?with COX4I1? Monoclonal Antibody (bsm-33037R-HRP) at 1:200 overnight at 4°C, and?DAB?(C-0010)?staining.
Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The?section?was?incubated?with COX4I1? Monoclonal Antibody (bsm-33037R-HRP) at 1:200 overnight at 4°C, and?DAB?(C-0010)?staining.
Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The?section?was?incubated?with COX4I1? Monoclonal Antibody (bsm-33037R-HRP) at 1:200 overnight at 4°C, and?DAB?(C-0010)?staining.
Paraformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The?section?was?incubated?with COX4I1? Monoclonal Antibody (bsm-33037R-HRP) at 1:200 overnight at 4°C, and?DAB?(C-0010)?staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The?section?was?incubated?with COX4I1? Monoclonal Antibody (bsm-33037R-HRP) at 1:200 overnight at 4°C, and?DAB?(C-0010)?staining.
Paraformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The?section?was?incubated?with COX4I1? Monoclonal Antibody (bsm-33037R-HRP) at 1:200 overnight at 4°C, and?DAB?(C-0010)?staining.
Paraformaldehyde-fixed, paraffin embedded Rat Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The?section?was?incubated?with COX4I1? Monoclonal Antibody (bsm-33037R-HRP) at 1:200 overnight at 4°C, and?DAB?(C-0010)?staining.
Paraformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The?section?was?incubated?with COX4I1? Monoclonal Antibody (bsm-33037R-HRP) at 1:200 overnight at 4°C, and?DAB?(C-0010)?staining.
Paraformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The?section?was?incubated?with COX4I1? Monoclonal Antibody (bsm-33037R-HRP) at 1:200 overnight at 4°C, and?DAB?(C-0010)?staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
