| 產(chǎn)品編號 | bs-22944R |
| 英文名稱 | Tartrate Resistant Acid Phosphatase Rabbit pAb |
| 中文名稱 | 抗酒石酸酸性磷酸酶5型/5型酸性磷酸酶抗體 |
| 別 名 | Type 5 acid phosphatase; Acid phosphatase 5 tartrate resistant; ACP5; PPA5_HUMAN; T5ap; Tartrate resistant acid ATPase; Tartrate resistant acid phosphatase type 5; Tartrate resistant acid phosphatase type 5 precursor; Tartrate-resistant acid ATPase; Tartrate-resistant acid phosphatase type 5; TR AP; TR-AP; TrATPase; Type 5 acid phosphatase. |
| 研究領(lǐng)域 | 腫瘤 細(xì)胞生物 免疫學(xué) 信號轉(zhuǎn)導(dǎo) |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應(yīng) | Human (predicted: Mouse,Rat) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 34 kDa |
| 檢測分子量 | |
| 細(xì)胞定位 | 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Tartrate Resistant Acid Phosphatase : 211-310/325 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Tartrate-resistant acid phosphatase is an iron containing glycoprotein that catalyzes the conversion of orthophosphoric monoester to alcohol and orthophosphate. TRAP is the most basic of the acid phosphatases and is the only form not inhibited by L+-tartrate. TRAP is a relatively minor lysosomal enzyme which may be activated in certain pathologies such as Hodgkin’s disease and B- and T-cell leukemias. Receptor activator of NFκB ligand (RANKL) plays an essential role in osteoclast differentiation and activation by increasing the expression of protease osteoclast markers such as TRAP. TRAP has collagenolytic activity and plays a major role in ligament degradation. Function: Involved in osteopontin/bone sialoprotein dephosphorylation. Its expression seems to increase in certain pathological states such as Gaucher and Hodgkin diseases, the hairy cell, the B-cell, and the T-cell leukemias. Subunit: Exists either as monomer or, after proteolytic processing, as a dimer of two chains linked by disulfide bond(s). Subcellular Location: Lysosome. DISEASE: Defects in ACP5 are the cause of spondyloenchondrodysplasia with immune dysregulation (SPENCDI) [MIM:607944]. A disease characterized by vertebral and metaphyseal dysplasia, spasticity with cerebral calcifications, and strong predisposition to autoimmune diseases. The skeletal dysplasia is characterized by radiolucent and irregular spondylar and metaphyseal lesions that represent islands of chondroid tissue within bone. Note=ACP5 inactivating mutations result in a functional excess of phosphorylated osteopontin causing deregulation of osteopontin signaling and consequential autoimmune disease. Similarity: elongs to the metallophosphoesterase superfamily. Purple acid phosphatase family. SWISS: P13686 Gene ID: 54 Database links: Entrez Gene: 54 Human Entrez Gene: 11433 Mouse Omim: 171640 Human SwissProt: P13686 Human SwissProt: Q05117 Mouse Unigene: 1211 Human Unigene: 46354 Mouse Unigene: 171928 Rat 抗酒石酸鹽酸性磷酸酶(Tartrate-Resistant Acid Phosphatase,TRAP)是破骨細(xì)胞功能的重要標(biāo)志物,它的活性與破骨細(xì)胞活性呈正相關(guān). |
| 產(chǎn)品圖片 |
Sample:
Lane 1: U251 (Human) Cell Lysate at 30 ug
Lane 2: A549 (Human) Cell Lysate at 30 ug
Lane 3: HepG2 (Human) Cell Lysate at 30 ug
Primary: Anti-Tartrate Resistant Acid Phosphatase (bs-22944R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kD
Observed band size: 36 kD
|
