| 產品編號 | bs-8854R |
| 英文名稱 | KLF3/ZNF741 Rabbit pAb |
| 中文名稱 | 鋅指蛋白741抗體 |
| 別 名 | Basic krueppel-like factor; Basic Kruppel like factor; BKLF3; CACCC box binding protein BKLF; CACCC-box-binding protein BKLF; Klf3; KLF3_HUMAN; Krueppel-like factor 3; Kruppel like factor 3(basic); MGC48279; TEF-2; TEF2; Transcript ch138; Zinc finger protein 741; ZNF741. |
| 研究領域 | 細胞生物 轉錄調節(jié)因子 表觀遺傳學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | (predicted: Human,Mouse,Rat,Sheep,Chicken,Dog,Horse) |
| 產品應用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC/IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 39 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human KLF3/ZNF741: 251-345/345 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
KLF3 is a transcriptional repressor and activator. It also binds the GT-box of cyclin D1 promoter and mediates cell cycle progression at G phase as a downstream target of focal adhesion kinase (FAK). Ubiquitously expressed.
Kruppel-like factor proteins all contain a characteristic C-terminal DNA binding domain containing 3 Kruppel-like zinc fingers, and they all recognize CACCC and GC boxes present in promoters and enhancers. Function: Binds to the CACCC box of erythroid cell-expressed genes. May play a role in hematopoiesis. Subcellular Location: Nucleus. Post-translational modifications: Sumoylated by SUMO1. Sumoylation is enhanced by PIAS1, PIAS2alpha and PIAS2beta, and PIAS4, but not by Pc2. Enhances transcriptional repression, but has no effect on DNA binding. Sumoylation on Lys-198 is the major site. Similarity: Belongs to the krueppel C2H2-type zinc-finger protein family. Contains 3 C2H2-type zinc fingers. SWISS: P57682 Gene ID: 51274 Database links: Entrez Gene: 488836 Human Entrez Gene: 51274 Human Entrez Gene: 16599 Mouse Omim: 609392 Human SwissProt: P57682 Human SwissProt: Q60980 Mouse Unigene: 298658 Human Unigene: 392759 Mouse Unigene: 439720 Mouse Unigene: 23326 Rat |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
