| 產(chǎn)品編號 | bs-6099R |
| 英文名稱 | SASH1 Rabbit pAb |
| 中文名稱 | 腫瘤抑制基因PEPE1抗體 |
| 別 名 | PEPE 1; PEPE1; Proline glutamate repeat containing protein; Proline-glutamate repeat-containing protein; RP3 323M4.1; SAM and SH3 domain containing 1; SAM and SH3 domain containing protein 1; SAM and SH3 domain-containing protein 1; SASH 1; Sash1; SASH1_HUMAN. |
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Specific References (3) | bs-6099R has been referenced in 3 publications.
[IF=7.035] Siyi Liu. et al. Depletion of SASH1, an astrocyte differentiation-related gene, contributes to functional recovery in spinal cord injury. CNS NEUROSCI THER. 2022 Oct;: WB ; Rat.
[IF=4.067] Cui HZ et al. SASH1 promotes melanin synthesis and migration via suppression of TGF-β1 secretion in melanocytes resulting in pathologic hyperpigmentation. International Journal of Biological Sciences. 2020; 16(7): 1264-1273. IHC-P&WB ; Human.
[IF=2.705] Ma C et al. Exosomal and extracellular HMGB1 have opposite effects on SASH1 expression in rat astrocytes and glioma C6 cells. Biochem Biophys Res Commun. 2019 Oct 15;518(2):325-330. WB ; Rat.
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| 研究領域 | 腫瘤 免疫學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | (predicted: Human,Mouse,Rat,Rabbit,Cow,Chicken,Dog,Horse) |
| 產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 137 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SASH1: 421-520/1247 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
SASH1 is a 1247 amino acid protein that is significantly downregulated in the majority of primary breast tumor tissues, breast cancer cell lines, lung and thyroid tumors, as well as in certain colon carcinomas. It has been hypothesized that its expression is suppressed not due to mutation of the SASH1 gene, but instead via other mechanisms, such as promoter methylation. As a member of the SH3-domain containing expressed in lymphocytes (SLY1) gene family, SASH1 contains two sterile α modules (SAMs) and one Src homology-3 (SH3) domain, motifs that are predominantly found in adaptors, scaffold proteins and signaling molecules. Downregulation of SASH1 expression correlates with the formation of distant metastasis and is considered a negative prognostic parameter for patient survival. Function: May have a role in a signaling pathway. Could act as a tumor suppressor. Tissue Specificity: Expressed ubiquitously, with highest levels in lung, placenta, spleen and thymus. Down-regulated in the majority (74%) of breast tumors in comparison with corresponding normal breast epithelial tissues. Similarity: ontains 2 SAM (sterile alpha motif) domains. Contains 1 SH3 domain. SWISS: O94885 Gene ID: 23328 Database links: Entrez Gene: 23328 Human Entrez Gene: 70097 Mouse Omim: 607955 Human SwissProt: O94885 Human SwissProt: P59808 Mouse Unigene: 193133 Human Unigene: 338275 Mouse |
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
