| 產(chǎn)品編號 | bs-3215R |
| 英文名稱 | phospho-HDAC6 (Ser22) Rabbit pAb |
| 中文名稱 | 磷酸化組蛋白去乙?；?抗體 |
| 別 名 | HDAC6(phospho S22); p-HDAC6(phospho S22); HDAC6(phospho Ser22); p-HDAC6(Ser22); HD 6; HD6; HDAC 6; Histone deacetylase 6; HD6; Histone deacetylase 6; JM 21; JM21; KIAA0901; FLJ16239; HDAC6_HUMAN. |
| 產(chǎn)品類型 | 磷酸化抗體 |
| 研究領域 | 腫瘤 免疫學 發(fā)育生物學 信號轉導 細胞凋亡 轉錄調節(jié)因子 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human |
| 產(chǎn)品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 134 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 細胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human HDAC6 around the phosphorylation site of Ser22: PQ(p-S)PP |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
HDAC6 is a member of the class II mammalian histone deacetylases. Human HDAC6 is composed of 1215 amino acid residues. It possesses two separate putative catalytic domains. Both catalytic domains are fully functional HDACs and contribute independently to the overall activity of HDAC6 protein. A very potent NES is present at the amino-terminus of HDAC6, which was found to play an important role in regulating the shuttling of HDAC6 protein between cytoplasm and nucleus. The shuttling process may be a critical regulatory mechanism of HDAC6 function. The expression of HDAC6 is tightly linked to the state of cell differentiation. HDAC6 may participate in coordinating expression of a group of genes involved in the remodelling of chromatin during cell differentiation. Function: Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin. In addition to its protein deacetylase activity, plays a key role in the degradation of misfolded proteins: when misfolded proteins are too abundant to be degraded by the chaperone refolding system and the ubiquitin-proteasome, mediates the transport of misfolded proteins to a cytoplasmic juxtanuclear structure called aggresome. Probably acts as an adapter that recognizes polyubiquitinated misfolded proteins and target them to the aggresome, facilitating their clearance by autophagy. Subunit: Interacts with CBFA2T3, HDAC11 and SIRT2. Interacts with F-actin. Interacts with BBIP10. Under proteasome impairment conditions, interacts with UBD via its histone deacetylase 1 and UBP-type zinc-finger regions. Interacts with CYLD. Interacts with ZMYND15 (By similarity). Interacts with DDIT3/CHOP. Subcellular Location: Nucleus. Cytoplasm. Note=It is mainly cytoplasmic, where it is associated with microtubules. Post-translational modifications: Phosphorylated by AURKA. Ubiquitinated. Its polyubiquitination however does not lead to its degradation. Sumoylated in vitro. Similarity: Belongs to the histone deacetylase family. HD type 2 subfamily. Contains 1 UBP-type zinc finger. SWISS: Q9UBN7 Gene ID: 10013 Database links: Entrez Gene: 10013 Human Entrez Gene: 15185 Mouse Omim: 300272 Human SwissProt: Q9UBN7 Human SwissProt: Q9Z2V5 Mouse Unigene: 6764 Human Unigene: 29854 Mouse Unigene: 13453 Rat ????HDAC6是一種比較獨特的組蛋白去乙酰化酶，含有兩個功能上相互獨立的HDAC催化結構域。HDAC6可以去乙酰化組蛋白并抑制相關基因轉錄。 ????HDAC6可以和微管(microtuble)結合，可以去乙酰化tubulin，Hsp90和cortactin等。目前發(fā)現(xiàn)大量的蛋白可以被乙?；揎棧虼薍DAC等乙?；揎椕副徽J為在基因轉錄調控、信號轉導、生長發(fā)育、分化凋亡、代謝性疾病和腫瘤等多種生理病理過程中發(fā)揮重要作用。HDAC的抑制劑目前被認為是很有前景的腫瘤治療藥物。 ????內源性HDAC6主要定位于細胞漿，與微管相結合并且是一個微管蛋白去乙酰化酶。HDAC6含有一個鋅指結構域，該結構域可能和泛素化降解的調節(jié)有關。HDAC6可以和DHAC11相互作用。 |
| 產(chǎn)品圖片 |
Sample:
Jurkat(Human) Cell Lysate at 30 ug
K562(Human) Cell Lysate at 30 ug
Primary: Anti-phospho-HDAC6 (Ser22) (bs-3215R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 134 kD
Observed band size: 134 kD
Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HDAC6 (Ser22)) Polyclonal Antibody, Unconjugated (bs-3215R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
|
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
