| 產品編號 | bs-1299R |
| 英文名稱 | Parvalbumin Rabbit pAb |
| 中文名稱 | 細小清蛋白抗體 |
| 別 名 | D22S749; MGC116759; Parvalbumin alpha; PRVA_HUMAN; PVALB. |
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Specific References (2) | bs-1299R has been referenced in 2 publications.
[IF=2.21] Qi et al. Interaction of basolateral amygdala, ventral hippocampus and medial prefrontal cortex regulates the consolidation and extinction of social fear. (2018) Behav.Brain.Funct. 14:7 other ; Mouse.
[IF=1.68] Chen J et al. Morphology of Inner Retina in Rhesus Monkeys of Various Ages: A Comparative Study.J Ophthalmol. 2019 Mar 3;2019:7089342. IF ; Monkey.
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| 研究領域 | 神經生物學 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號 | |
| 交叉反應 | Human,Mouse,Rat (predicted: Pig,Cow,Chicken,Horse) |
| 產品應用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 12 kDa |
| 檢測分子量 | |
| 細胞定位 | 細胞核 細胞外基質 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Parvalbumin: 7-110/110 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產品介紹 |
The protein encoded by this gene is a high affinity calcium ion-binding protein that is structurally and functionally similar to calmodulin and troponin C. The encoded protein is thought to be involved in muscle relaxation. Belongs to the parvalbumin family. Contains 2 EF-hand domains. This protein binds two calcium ions. Function: In muscle, parvalbumin is thought to be involved in relaxation after contraction. It binds two calcium ions. Similarity: Belongs to the parvalbumin family. Contains 2 EF-hand domains. SWISS: P20472 Gene ID: 5816 Database links: Entrez Gene: 396459 Chicken Entrez Gene: 396531 Chicken Entrez Gene: 5816 Human Entrez Gene: 19293 Mouse Omim: 168890 Human SwissProt: P20472 Human SwissProt: P32848 Mouse Unigene: 295449 Human Unigene: 2766 Mouse Unigene: 2005 Rat (Parvalbumin, PV)細小白蛋白屬于鈣結合蛋白家族,是一種細胞內可溶的小結合蛋白，過去曾認為，僅出現在哺乳動物某些肌纖維內,它與Ca2+有高度親合力,與鈣調蛋白競爭自由Ca2+,故可阻止肌漿內的Ca2+增高，可抗衡細胞內鈣過量的發(fā)生。 |
| 產品圖片 |
Sample:
Lane 1: Mouse Kidney tissue lysates
Lane 2: Rat Kidney tissue lysates
Primary: Anti-Parvalbumin (bs-1299R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 12 kDa
Observed band size: 10 kDa
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (Parvalbumin) Polyclonal Antibody, Unconjugated (bs-1299R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (Parvalbumin) Polyclonal Antibody, Unconjugated (bs-1299R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
