| 產(chǎn)品編號(hào) | bs-1101R |
| 英文名稱 | MCP1 Rabbit pAb |
| 中文名稱 | 單核細(xì)胞趨化蛋白1抗體 |
| 別 名 | MCP-1/CCL2; C-C motif chemokine 2; CCL 2; CCL2; CCL2_HUMAN; Chemokine(C C motif) ligand 2; Chemokine C C motif ligand 2; Chemokine CC Motif Ligand 2; GDCF 2; GDCF 2 HC11; GDCF-2; GDCF2; HC11; HSMCR30; HSMCR30; JE; MCAF; MCP 1; MCP-1; MGC9434; Monocyte chemoattractant protein 1; Monocyte chemotactic and activating factor; Monocyte chemotactic protein 1; Monocyte secretory protein JE; SCYA2; Small inducible cytokine A2(monocyte chemotactic protein 1, homologous to mouse Sig je); Small inducible cytokine A2; Small inducible cytokine subfamily A(Cys Cys), member 2; Small inducible cytokine subfamily A Cys Cys member 2; Small-inducible cytokine A2; SMC CF; SMC-CF; SMCCF. |
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Specific References (19) | bs-1101R has been referenced in 19 publications.
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| 研究領(lǐng)域 | 腫瘤 免疫學(xué) 轉(zhuǎn)錄調(diào)節(jié)因子 |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Polyclonal |
| 克 隆 號(hào) | |
| 交叉反應(yīng) | Rat |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:400-800,IHC-F=1:400-800,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 11 kDa |
| 檢測(cè)分子量 | |
| 細(xì)胞定位 | 分泌型蛋白 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from rat MCP-1: 61-148/148 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. Monomer or homodimer; in equilibrium. Binds to CCR2 and CCR4. Is tethered on endothelial cells by glycosaminoglycan (GAG) side chains of proteoglycans. Processing at the N-terminus can regulate receptor and targetcell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant. Genetic variations in CCL2 determine Mycobacterium tuberculosis susceptibility Belongs to the intercrine beta (chemokine CC) family. The MCP-1( the monocyte chemotactic protein1) has many pits that regulates the function toward sex to turn to record the factor, it can induce to have relation with inflammation with the immunity various genes turn to record.The immunity MCP-1 set turns the male signal to mainly locate the afterbirth syrup inside. Function: Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. Subunit: Monomer or homodimer; in equilibrium. Binds to CCR2 and CCR4. Is tethered on endothelial cells by glycosaminoglycan (GAG) side chains of proteoglycans. Subcellular Location: Secreted. Post-translational modifications: Processing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant. Similarity: Belongs to the intercrine beta (chemokine CC) family. SWISS: P14844 Gene ID: 24770 Database links: Entrez Gene: 6347 Human Entrez Gene: 20296 Mouse Omim: 158105 Human SwissProt: P13500 Human SwissProt: P10148 Mouse Unigene: 303649 Human Unigene: 290320 Mouse Unigene: 4772 Rat 生長(zhǎng)因子和激素（ Growth Factor and Hormones） 巨噬細(xì)胞趨化蛋白1(MCP1)具有多向性調(diào)節(jié)作用的核轉(zhuǎn)錄因子，它可誘導(dǎo)與免疫和炎癥有關(guān)的多種基因轉(zhuǎn)錄.MCP-1免疫組化陽(yáng)性信號(hào)主要位于胞漿內(nèi)。 單核細(xì)胞趨化蛋白-1（monocyte chemotactic protein 1,MCP-1)又稱單核細(xì)胞趨化和激活因子（monoctye chemotactic and activating factor,MCAF)，屬于C-C亞族（β亞族）成員。MCP-1/MCAF起初是從人髓樣單核細(xì)胞系THP-1、神經(jīng)膠質(zhì)瘤細(xì)胞系以及LPS或PHA刺激的人PBMC培養(yǎng)上清中發(fā)現(xiàn)。目前已知，單核細(xì)胞、巨噬細(xì)胞、成纖維細(xì)胞、內(nèi)皮細(xì)胞、B細(xì)胞、平滑肌細(xì)胞等在PHA、LPS、Poly I-C、IL-1、IFN-γ、PDGF、EGF或某些病毒刺激下均可被誘導(dǎo)分泌MCP-1。某些腫瘤細(xì)胞系則可組成性產(chǎn)MCP-1。 |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MCP1) Polyclonal Antibody, Unconjugated (bs-1101R ) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: mouse kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-MCP-1 Polyclonal Antibody, Unconjugated(bs-1101R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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