| 產(chǎn)品編號 | bsm-62970R |
| 英文名稱 | Phospho-RPS6KB1 (Thr421/Ser424) Recombinant Rabbit mAb |
| 中文名稱 | 磷酸化核糖體S6蛋白激酶重組兔單抗 |
| 別 名 | EC 2.7.11.1; KS6B1; P70-S6K; RPS6KB1; Ribosomal protein S6 kinase; Ribosomal protein S6 kinase; 70kDa; polypeptide 1; S6K; kinase p70S6K; p70-S6K. |
| 抗體來源 | Rabbit |
| 克隆類型 | Recombinant |
| 克 隆 號 | 18F4 |
| 交叉反應 | Human,Rat |
| 產(chǎn)品應用 | WB=1:500-1:2000,IP=1:20-1:50
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 59 kDa |
| 檢測分子量 | 70 |
| 性 狀 | Liquid |
| 免 疫 原 | A synthesized peptide derived from human p70 S6 Kinase around the phosphorylation site of T421/S424: PR-pT-PV-pS-PV |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 10mM phosphate buffered saline(pH 7.4) with 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol. |
| 保存條件 | Store at 4℃ for short term. Store at -20℃ for long term. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Acts to integrate nutrient and growth factor signals in regulation of protein synthesis, cell proliferation, cell growth, cell cycle progression and cell survival. Downstream effector of the mTOR signaling pathway. SWISS: P23443 Gene ID: 6198 |
| 產(chǎn)品圖片 |
25 ug total protein per lane of various lysates (see on figure) probed with Phospho-RPS6KB1 monoclonal antibody, unconjugated (bsm-62970R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
